FluoPi 2
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FluoPi 2 Basic Data Category: Microscopes URL (first publication): https://www.biomaker.org/projects/open-source-autonomous-imaging-station Keywords: Microscope License: Unlicense Project status: Active
Technical documentation Maturity of the project: production / DIY
CAD files are editable: Yes Assembly instructions are published: Yes Bill of materials is published: Yes
yes yes
Project management
Open-o-meter: 7 Product category: Cameras & Optics
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Description
We proposed to create a set of tools for scientific research, engineering and teaching biology at the scale of whole colonies and entire plates. First, we designed and built a standalone tool for imaging and analysing fluorescence in biological samples at three different spectra and at a range of scales from individual colonies to whole plates (easily extensible to plant cells and even whole organisms such as C. elegans and Marchantia spp). The system is self-contained and autonomous, including hardware and software for image capture, programmed sequences (e.g. timelapse), and quantitative analysis of samples. We also developed a simple genetic toolkit for the production of fluorescent and pigmented bacteria complementing the device. We have optimized genetic construction protocols and adopted fluorescent markers that can be used with 470 nm single excitation. Altogether, our system allows for construction and integral experimentation with hardware, software and genetic instructions from off-the-shelve, open access and distributed resources.
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